How do you draw blood from a mouse?

So-called “terminal” or “non-survival” blood collection is done either pre- or post-mortem and only after the mouse has received surgical anesthesia. Terminal collection can be taken via cardiac puncture and can collect the maximum amount of blood. It can also be done via the aorta or vena cava.

Which is the most common blood collection method in mice?

Blood Collection (Guideline)

Species Common Blood Collection Route(s)
Mouse Submandibular vein
Tail vein
Saphenous vein
Retro-orbital sinus (see below)

Which method is used to obtain blood from rodents?

Mice & Rats – Orbital Sinus The Orbital Sinus method may be used to collect large volumes of blood. Appropriate anesthesia must be used for this procedure. Animals may receive topical anesthetic (i.e., tetracaine ophthalmic drops) or general anesthesia.

What are the different methods of performing venipuncture?

Venipuncture can take place in a general medical practitioner’s office and is often carried out by a trained phlebotomist or nurse….Three popular methods of blood collection are:

  • Arterial Sampling.
  • Venipuncture Sampling.
  • Fingerstick Sampling.

How much blood can you extract from a mouse?

Without fluid replacement, approximately 0.007-0.008 ml of blood/gm BW can be safely withdrawn (about 0.18-0.2 ml of blood from a 25-gm mouse); with fluid replacement, approximately 0.014-0.016 ml blood/gm BW can be withdrawn (about 0.35-0.4 ml blood from a 25-gm mouse).

How much blood volume is in a mouse?

about 1.5-2.5 ml
An adult mouse has a circulating blood volume of about 1.5-2.5 ml (6-8% of the body weight), however in older and obese animals this may be lower.

What is the blood volume of a mouse?

77-80 ml/kg
The total blood volume of a mouse is between 77-80 ml/kg of body weight (bwt). Here is an example: A mouse weighs 25 grams and a single sample of blood is needed.

How often can you bleed a mouse?

Ideally, if a mouse will have large amounts (i.e., approaching the recommended maximum) of blood withdrawn more than three times at intervals of two weeks or less, its hematocrit and/or hemoglobin should be monitored. If deficits are found in these parameters, sampling volume and/or frequency should be reduced.

How do you extract blood from animals?

The animal is restrained manually or using a suitable animal restrainer. Hind leg is immobilized and slight pressure may be applied gently above the knee joint. The vein is punctured using a 20G needle and enough volume of blood is collected with a capillary tube or a syringe with a needle.

What is the most common patient concern when having blood drawn?

Getting blood drawn is a simple process, but the most common complication associated with it is bruising. Your doctor might call this kind of bruise is also called a hematoma.

Which sites should you avoid for venipuncture?

Certain areas are to be avoided when choosing a site: Extensive scars from burns and surgery – it is difficult to puncture the scar tissue and obtain a specimen. The upper extremity on the side of a previous mastectomy – test results may be affected because of lymphedema. Hematoma – may cause erroneous test results.

How often can I bleed mice?

What do you need to know about venipuncture?

This is why a required skill for most phlebotomy jobs is to have excellent people skills with the ability to keep the patient calm during the venipuncture procedure. Venipuncture can also happen when patients have intravenous (IV) lines started.

Can a phlebotomist switch sites during venipuncture?

In some cases, a phlebotomist may have to switch sites if they find the blood is not properly flowing from the vein. It is not uncommon for adults and children to feel some fear when having blood drawn, which is why any discussion of venipuncture should at least bring this topic to focus.

How long does it take to clean a venipuncture?

Apply the tourniquet 3-4 inches above the collection site. Never leave the tourniquet on for over 1 minute. If a tourniquet is used for preliminary vein selection, release it and reapply after two minutes. 7. Clean the puncture site by making a smooth circular pass over the site with the 70%

Do you need a discard tube for venipuncture?

NOTE: When using a winged blood collection set for venipuncture and a coagulation tube is the first tube needed, first draw a discard tube (plain red top or light blue top). The discard tube does not need to be filled completely.