How do you find the partition coefficient of benzoic acid?
log Caq
- Vaq= Volume in ml of 0.01N Sodium hydroxide per 10ml of aqueous layer.
- Naq= Normality of aqueous layer.
- Caq= Concentration of aqueous layer in g mole/lit = Normality (Naq)
- K= Caq/Corg½= Partition coefficient of benzoic acid in water and benzene.
Which are the methods for determination of partition coefficient?
Three experimental techniques were employed to determine the partition coefficient (K) of toluene, d-limonene, and ethyl acetate between water and polystyrene. These techniques were gas chromatography, (GC) gel permeation chromatography (a novel approach), and a dynamic thermal stripping-thermal desorption procedure.
What are the limitations of distribution coefficient?
The solute that is being distributed shall not on ant condition reactive towards the solvents being used. The molecular state of the solute must remain constant when in contact with the solvent. It should not undergo dissociation or association.
Why is the partition coefficient important?
The blood:gas partition coefficient is an important determinant of the speed of anesthetic induction and recovery. It describes the partition of an agent between a gaseous phase, such as alveolar air, and the blood. The greater the blood:gas partition coefficient, the greater the solubility in blood.
What are the factors affecting distribution coefficient?
Factors affecting Distribution Coefficient- The value of K depends upon the temperature , nature of solute , nature of two solvents . It does not depend upon the amount of solute or solvents taken.
What is distribution law and its limitations?
Limitations of the Nernst Distribution Law: The law is valid when the molecular state of the solute is same in both the solvents. If the solute undergoes dissociation or association in any one of the solvents, then in such cases the distribution law no longer holds good.
How to calculate the distribution coefficient of benzoic acid?
First extraction: in a sep funnel add 50.0 mL of the aqueous benzoic acid solution and 10.0 mL dichloromethane (DCM). Shake and drain off the lower DCM layer. Now titrate the aqueous layer with NaOH to determine how much benzoic acid remained in the water.
How to calculate KD from benzoic acid extraction?
Using this data you can calculate Kd. Second extraction: extract 50.0 mL aqueous benzoic acid with two 5.0 mL portions of DCM. After the second extraction drain off the lower DCM layer and once again titrate the aqueous layer to determine how much benzoic acid remains.
Is the partition of benzoic acid always the same?
In this experiment, the partition coefficient for this method of extraction would be the same regardless of which method of extraction is used.
Which is the more efficient way to extract benzoic acid?
So if we have a solution of benzoic acid in water, which is the more efficient way to extract it: doing a single extraction using 10 mL of DCM, or two extractions, each using 5 mL of DCM? By the rule “the correct answer will keep you in orgo lab longer”, it’s the second choice- two 5 mL extractions.