Where did HEK293 cells come from?
The HEK293 cell line was initially produced in 1973 by a team led by Alex van der Eb in Leiden (Netherlands) from normal fetal human embryonic kidney cells.
Are HEK cells cancer cells?
HEK 293 cells are popular for their ease of growth and transfection, making them a common cell culture in cancer research. HEK 293 can be employed as a highly malignant tumor model as its tumorigenicity increases significantly in high-passage.
What are HEK cells used for?
HEK 293 cells have been widely used in cell biology research for many years, because of their reliable growth and propensity for transfection. They are also used by the biotechnology industry to produce therapeutic proteins and viruses for gene therapy.
What are FreeStyle cells?
FreeStyle 293-F cells are derived from HEK293 cells; they were just cloned extensively for growth, viability, and protein production in the new serum-free FreeStyle 293 medium.
Are HEK293 cells immortal?
Thus, HEK293 is not a model for normal human cells; these cells are immortalized already by known oncogene but not malignant yet.
Can HEK293 cause cancer?
Among them, only the immortalized human embryonic kidney cell line (HEK293) was prone to malignant transformation following exposure to cancer patient serum. These cells are generated by culture with Adenovirus 5 DNA that results in the insertion of approximately 4.5 kb into chromosome 19 [22].
Are HEK293 normal cells?
[5] in early 70s by transformation of culture of normal human embryonic kidney cells with sheared human adenovirus type 5 DNA. Thus, HEK293 is not a model for normal human cells; these cells are immortalized already by known oncogene but not malignant yet.
How big are HeLa cells?
20 ~ 40 µm
HeLa cells are normally 20 ~ 40 µm in diameter depending on the culture conditions. Red blood cells, one of the smallest human cells, have a diameter of around 8 µm.
What is the difference between 293T and 293 ft?
293T cells and 293FT cells grow faster and are easier to transfect than HEK293 cells and result in higher titers of lentivirus. So most lentiviral protocols use 293FT cells to produce lentivirus.
How do you Lyse HEK293 cells?
Popular Answers (1)
- Pellet cells, resuspend in buffer, sonicate gently for 1-2 min (much less power needed than for E. coli). Then ultracentrifuge to pellet crude membranes.
- Directly solubilise the protein out of membranes with detergent (often DDM, CHAPSO or digitonin in our case, but this is protein dependent).
Why are HEK 293 cells used in cell biology?
HEK 293 cells have been widely used in cell biology research for many years, because of their reliable growth and propensity for transfection.
How many times has Graham transfected HEK 293 cells?
Graham performed the transfection a total of eight times, obtaining just one clone of cells that were cultured for several months. After presumably adapting to tissue culture, cells from this clone developed into the relatively stable HEK 293 line.
What is the band in HEK 293 FLP cells?
The western blot shows a band of approximately 70 kDa in HEK 293 Flp-In T-Rex FLAG-β 2 AR cells that were incubated with tetracycline for 24 h. A similar band is present in the positive control lane with cells expressing FLAG-β 2 AR constitutively. Data are representative of three individual experiments.
How often does a HEK293 cell line occur?
HEK293 is a hypotriploid cell line with modal chromosome number of 64, which occurs in 30% of cells. Cells with higher ploidies occur less frequently at a rate of 4.2%. HEK293 cell lines display some cytogenetic instability.