What is DEAE Sepharose chromatography?
DEAE Sepharose Fast Flow is a weak anion exchange chromatography resin (medium) for fast protein purification. Well-proven weak anion exchanger developed for industrial downstream processes. Used extensively for capture and intermediate purification of a wide range of approved biopharmaceuticals.
What is DEAE Sepharose Fast Flow?
DEAE Sepharose Fast Flow is a weak anion exchanger based on the well established Sepharose® Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.
What is DEAE ion exchange chromatography?
Diethylaminoethyl cellulose (DEAE-C) is a positively charged resin used in ion-exchange chromatography, a type of column chromatography, for the separation and purification of proteins and nucleic acids.
What is a DEAE column?
SMT DEAE [Di-Ethyl-Amino-Ethyl] column provides a unique chemically attached hydrophilic, weak anion exchange type, functional surface desirable for the separation of many biomolecules such as proteins, nucleotides, oligonucleotides, polynucleotides, high molecular weight RNA’s and plasmid DNA’s.
What is the full form of DEAE Sepharose?
DEAE-Sepharose is a tradename for the anion-exchange reactive group, diethylaminoethanol (DEAE) covalently linked to Sepharose (a polysaccharide polymer).
What is Sepharose used for?
Sepharose/agarose, combined with some form of activation chemistry, is also used to immobilize enzymes, antibodies and other proteins and peptides through covalent attachment to the resin.
Is DEAE cellulose anion or cation exchanger?
DEAE-cellulose contains an diethylaminoethyl group. It is positively charged at neutral pH and so DEAE-cellulose is a weak anion exchanger. The Sepharose types are particularly useful for the separation of high molecular weight proteins.
Is carboxymethyl cellulose positive or negative?
Protein that have a low density of net positive charge will tend to emerge first, followed by those having a higher charge density. Positively charged complexes or proteins (cationic proteins) can be separated on negatively charged carboxymethyl-cellulose (CM-cellulose) columns.
How do I choose ion exchange resin?
(4) a resin shall be chosen according to the ingredients of the impurities in the original water. Such as more organic substances in the original water, or removal of the radius of the ion, should choose the larger diameter of the resin. Try to choose high-strength porous resin.
What is CM Sephadex?
CM Sephadex A-50 is a weak cation exchanger based on the well documented and well proven Sephadex base matrix. Sephadex ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix. These groups are attached to glucose units in the matrix by stable ether linkages.
What’s the meaning of Deae?
deae cellulose. [ n ] used for chromatography.
What is butyl Sepharose?
Butyl Sepharose High Performance is a member of the Cytiva range of HIC resins for intermediate purification and polishing of proteins. Butyl Sepharose High Performance is based on highly crosslinked, 34-µm agarose beads modified with aliphatic butyl groups via uncharged, chemically stable ether linkages.