What does MS-222 do?
Tricaine methanesulfonate (MS-222) is structurally similar to benzocaine, a typical anesthetic for anamniote vertebrates, but contains a sulfate moiety rendering this drug more hydrophilic. MS-222 is used as anesthetic in poikilothermic animals such as fish and amphibians.
How do you use MS-222?
MS222 can be administered via immersing the animal in a MS222 solution (i.e. water bath), spraying onto gills with an aerosol pump sprayer in large fish (anesthesia only), or injected directly into the lymph sacs (amphibians) or the coelomic cavity (amphibians and reptiles).
What is MS222?
The chemical tricaine methanesulfonate (MS222) is an acceptable agent for euthanasia in fish and aquatic amphibians. 1,2,7,9,10,16,18,27. The standard protocol for euthanasia of these species is immersion in a pH-neutralized solution for 15 to 30 min, followed by a secondary physical euthanasia method.
How do you dispose of MS-222?
While working off campus or in the field, MS-222 waste solution with a pH between 6-10 may be disposed of by flushing the solution down the drain to a sanitary sewer with excess water. Solid MS-222 (powder or tablets) should be returned to campus for disposal through the DRS waste program.
How do you make Tricaine solution?
To prepare 50 mL of stock solution, add 48 mL of H2O to 200 mg of Tricaine powder and 2 mL of 1 m Tris base (pH 9). Adjust to pH 7. Store at –20°C in 1-mL aliquots.
How does MS-222 work on fish?
MS-222 is effective and safe for the anesthesia of fish when used as directed. The action of the anesthetic is slowed at cooler temperatures, in extremely soft water (approximately 10 mg/liter of CaCO3 or less), and in larger fish. Additionally, efficacy may vary with size, species, and concentration of fish.
How do you use Tricaine as a buffer?
Tricaine is a popular anesthetic agent because of its solubility in water. However, because tricaine is acid in aqueous solution, it should be buffered. Most commonly this is achieved with bicarbonate of soda.
How do you dispose of benzocaine?
Liquid MS-222 or Benzocaine waste, such as tank water, can be disposed of via sanitary sewer if the liquid solution contains less than a 10% concentration of MS-222 or Benzocaine.
How do you euthanize an adult zebrafish?
For zebrafish 4-7 days post-fertilization (dpf) the following methods are acceptable for euthanasia: Immobilization by submersion in ice water (5 parts ice/1 part water, 0-4º C) for at least 20 minutes to ensure death by hypoxia.
When to use tricaine-s ( MS-222 ) for fish?
Tricaine-S (MS-222) is an FDA approved fish anesthetic used for the temporary immobilization of fish, amphibians, and other aquatic cold-blooded animals (poikilotherms). When used there is a 21 day withdrawal period. Aquacalm™ has been granted Indexed status by the FDA for the sedation and anesthesia of ornamental finfish.
Which is the best anesthetic for fish anesthesia?
TRICAINE-S (MS-222) is a fine crystalline powder fish anesthetic that is mixed with water prior to use. MS-222 is safe and effective for the anesthesia of fish when used as directed. Western Chemical is the world’s largest manufacturer of MS-222, with TRICAINE-S being produced in our FDA inspected Ferndale Washington USA manufacturing plant.
When to use MS-222 for finfish euthanasia?
MS-222 is also an acceptable method of euthanasia for finfish and for some amphibians and reptiles. When used for large finfish and some amphibians (e.g., Xenopus spp.), a secondary method should be used to ensure death (e.g. pithing with or without decapitation).
What do you need to know about finquel MS-222?
This page contains information on Finquel MS-222 (Tricaine Methanesulfonate) for veterinary use. Direction and dosage information for Finquel MS-222 (Tricaine Methanesulfonate) For Anesthesia and Tranquilization of Fishes and Other Cold-Blooded Animals