What is the difference between Sepharose and Sephadex?
Sephadex is a bead-formed gel prepared by cross-linking dextran with epichlorohydrin. Sepharose is a bead-formed gel prepared from agarose by a purification process that removes the charged polysaccharides and gives a gel with only a very small number of residual, charged groups (see Electroendosmosis ).
What is Sepharose 4B?
Sepharose® 4B is an agarose bead and a polymer of Gal β1,4 [3,6]-anhydro-L-galactose. Sepharose aids in fractionating larger molecules and is devoid of charged saccharides. Sepharose is widely used in affinity chromatography by cross linking various ligands on the matrix.
What is the binding capacity of DEAE Sephacel?
|Flow Rate||<50 cm/h|
|pH Range||2 – 12|
|Total Ionic Capacity||0.10–0.14 mmol Cl-/mL|
|Binding Capacity||10 mg Thyroglobulin (MW 669 000)/mL drained medium, 160 mg HSA (MW 68 000)/mL drained medium|
|Storage Temperature||4 – 8 °C|
What is G in Sephadex g25?
Sephadex G-25 is one of six different Sephadex G-types. The different types of Sephadex resins differ in their degree of cross-linking and hence in their degree of swelling and molecular fractionation range. Sephadex G-25 is available in four different particle size grades (Coarse, Medium, Fine, and Superfine).
What is Sepharose 6B?
Sepharose 6B is a well-proven agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.
What is DEAE column?
SMT DEAE [Di-Ethyl-Amino-Ethyl] column provides a unique chemically attached hydrophilic, weak anion exchange type, functional surface desirable for the separation of many biomolecules such as proteins, nucleotides, oligonucleotides, polynucleotides, high molecular weight RNA’s and plasmid DNA’s.
What is Blue Sepharose?
Blue Sepharose® 6 Fast Flow is composed of cross-linked 6% agarose beads modified with Cibacron Blue 3G covalently attached by the triazine coupling method. The blue dye binds many proteins, such as albumin, interferon, lipoproteins and blood coagulation factors.
What is the difference between sephadex and sepharose?
There is different type of Sepharose, best of all will be SP Sepharose High Performance, which has Particle Size 24 µm-44 µm while SP Sephadex C-50 has 40 µm-120 µm.
Which is better SP sepharex or SP Sephadex C-50?
There is different type of Sepharose, best of all will be SP Sepharose High Performance, which has Particle Size 24 µm-44 µm while SP Sephadex C-50 has 40 µm-120 µm. It’s mean that separation by of Sepharose High Performance will be better. But generally you can use your Sephadex saporin toxin.
What’s the difference between Cm Cellulose and sepharose?
The same applies for CM Cellulose and CM Sepharose, with CM standing for the carboxmethyl group a weak cation exchanger. Flow rates will differ as may column packing requirements to achieve comparable results, so you’ll have to try it out and follow the manufacturer’s recommendations.