What are micro cuvettes used for?
Plastic cuvettes are used in the visible range for colormetric assays and the UV range for DNA, RNA, and protein analysis. Semi-micro size cuvettes are for sample volumes between 1.5mL and 3.0mL, macro cuvettes for samples larger than 2.5mL.
What are the different types of cuvette cuvettes?
The four most popular cuvette materials are listed below:
- Optical Glass or Pyrex Glass.
- UV Quartz.
- IR Quartz.
What are the types of cuvette used in colorimeter?
What is the difference between the types of cuvettes you sell for Spectrometers and the Colorimeter?
- Quartz Cuvettes (CUV-QUARTZ) This is the best option for UV Range absorbance measurements, made of Far UV Quartz with a spectral range from 170 to 2700 nm.
- Fluorescence/UV Quartz Cuvette (CUV-QUARTZ-FUV)
Which is the most commonly used cuvette in spectrophotometry?
The most common type of cuvette is square, with external dimensions of 12.5 x 12.5 mm. This format accommodates sample volumes from the microliter range (ultra-micro cuvettes) to the milliliter range (macro cuvettes) (figure 1).
Does cuvette size affect absorbance?
The absorbance is directly proportional to the length of the light path (l), which is equal to the width of the cuvette.
How much does it cost to fill a cuvette?
Fill the cuvette about 3/4 full of the solution you wish to test. Wipe the outside of the cuvette with a lint-free, soft tissue (a Shurwipe or and Accuwipe) to remove any moisture or fingerprints from the outside surface.
Which detector is used in colorimeter?
a photoelectric detector that converts the reflected light intensity into an electrical output. Measurements made on a tristimulus colorimeter are normally comparative, the instrument being standardized on glass or ceramic standards.
Why quartz is used in cuvette?
Quartz. Quartz cells provide more durability than plastic or glass. Quartz excels at transmitting UV light, and can be used for wavelengths ranging from 190 to 2500 nm.