What stain is used to stain a cytospin slide?

Two types of stain methods were used in this study: Giemsa and methylene blue/eosin. For methylene blue/eosin staining, the slides were fixed by placing 2 drops of fixing solutions on the slides and air dried in a biological hood (2 min) or on bench (3–5 min).

How do you stain a Giemsa slide?

Staining procedure 1: Thin Film staining

  1. On a clean dry microscopic glass slide, make a thin film of the specimen (blood) and leave to air dry.
  2. dip the smear (2-3 dips) into pure methanol for fixation of the smear, leave to air dry for 30seconds.
  3. Flood the slide with 5% Giemsa stain solution for 20-30 minutes.

What Giemsa stain detects?

Giemsa stain makes it possible to demonstrate the presence of microorganisms in all types of tissues. It can especially be used to detect the presence of Helicobacter pylori in the diagnosis of gastric ulcers or chronic gastritis.

How does Giemsa stain work?

Giemsa solution is composed of eosin and methylene blue (azure). The eosin component stains the parasite nucleus red, while the methylene blue component stains the cytoplasm blue. The thin film is fixed with methanol. De-haemoglobinization of the thick film and staining take place at the same time.

Why do we use Giemsa stain?

Giemsa stain is used to obtain differential white blood cell counts. It is also used to differentiate nuclear and cytoplasmic morphology of the various blood cells like platelets, RBCs, WBCs. This stain is also used in cytogenetics to stain the chromosomes and identify chromosomal aberrations.

Why Giemsa stain is used in G banding?

G-banding, G banding or Giemsa banding is a technique used in cytogenetics to produce a visible karyotype by staining condensed chromosomes. Heterochromatic regions, which tend to be rich with adenine and thymine (AT-rich) DNA and relatively gene-poor, stain more darkly in G-banding.

Why is Giemsa stain used?

Giemsa stain is mainly used for staining of peripheral blood smears and specimens obtained from the bone marrow. It is used to obtain differential white blood cell counts. Giemsa stain is also used in cytogenetics to stain the chromosomes and identify chromosomal aberrations.

How to make a Giemsa stain on a glass slide?

On a clean dry microscopic glass slide, make a thin film of the specimen (blood) and leave to air dry. dip the smear (2-3 dips) into pure methanol for fixation of the smear, leave to air dry for 30seconds Flood the slide with 5% Giemsa stain solution for 20-30 minutes. Flush with tap water and leave to dry

How is the Giemsa stain used in cytogenetics?

Giemsa stain also is used to stain Histoplasma capsulatum, Pneumocystis jiroveci, Klebsiella granulomatis, Penicillium marneffei and occasionally bacterial capsules. This stain is also used in cytogenetics to stain the chromosomes and identify chromosomal aberrations.

How long do you put a Giemsa stain on a microscope?

A thin film of the specimen on a microscope slide is fixed in pure methanol for 30 seconds, by immersing it or by putting a few drops of methanol on the slide. The slide is immersed in a freshly prepared 5% Giemsa stain solution for 20–30 minutes (in emergencies 5–10 minutes in 10% solution can be used),…

How is the Giemsa stain used in G banding?

Giemsa stain is used in Giemsa banding (G-banding), to stain chromosomes and it is often used to create a diagrammatic representation of chromosomes (idiogram). Being a differential stain, Giemsa stain can be used to study the adherence of pathogenic bacteria to human cells, differentiating human cells as purple and bacterial cells as pink.