What is the function of CDC6 during S phase?
Cdc6 is also required during S phase to allow Chk1 activation in response to replication fork inhibition, though ORC-dependent chromatin binding is not required for this function.
What is the role of the CDC6 in replication?
Cell division cycle 6 (CDC6) is an essential regulator of DNA replication in eukaryotic cells. Its best-characterized function is the assembly of prereplicative complexes at origins of replication during the G(1) phase of the cell division cycle.
What does CDC6 and Cdt1 do?
CDC6 is an ATP binding protein and a member of the pre-replicative complex (pre-RC) together with the origin recognition complex (ORC), Cdt1 and the MCM complex (containing MCM2-7p). It is thought that the CDC6-Cdt1 complex uses ATP hydrolysis to thread DNA through the central hole of the MCM doughnut.
What happens when Cdc6 is phosphorylated?
In mammalian cells, phosphorylation of Cdc6 at the beginning of S phase causes its dissociation from chromatin and triggers replication. In addition, Cdc6 phosphorylation induces its nuclear export and ubiquitin-dependent degradation.
What is the function of CDT1?
The CDT1 gene provides instructions for making a protein that is important in the copying of a cell’s DNA before the cell divides (a process known as DNA replication). The protein produced from this gene is one of a group of proteins known as the pre-replication complex.
Why is Cdc6 important in helicase loading?
ATP hydrolysis by Cdc6 and ORC plays distinct and ordered roles in helicase loading. Cdc6 is activated to bind and hydrolyze ATP when it associates with origin-bound ORC, and this hydrolysis event is required to observe Mcm2–7 loading (Perkins and Diffley 1998; Randell et al. 2006).
What are the 4 stages of the cell cycle?
In eukaryotes, the cell cycle consists of four discrete phases: G1, S, G2, and M. The S or synthesis phase is when DNA replication occurs, and the M or mitosis phase is when the cell actually divides. The other two phases — G1 and G2, the so-called gap phases — are less dramatic but equally important.
Why is CDC6 important in helicase loading?
What does CDT1 stand for?
CDT1 (Chromatin Licensing And DNA Replication Factor 1) is a Protein Coding gene. Diseases associated with CDT1 include Meier-Gorlin Syndrome 4 and Meier-Gorlin Syndrome 1. Among its related pathways are Cell cycle Role of SCF complex in cell cycle regulation and CDK-mediated phosphorylation and removal of Cdc6.
What is the phase of mitosis?
Today, mitosis is understood to involve five phases, based on the physical state of the chromosomes and spindle. These phases are prophase, prometaphase, metaphase, anaphase, and telophase.
How is Cdc6 degraded in the cell cycle?
Cdc6 is an unstable protein that is rapidly degraded at the G1 to S phase transition (Piatti et al., 1996; Drury et al., 1997; Sánchez et al., 1999) and that must be synthesized de novo in each cell cycle.
What is the role of Cdc6 in mitosis?
However, CDC6 also plays important roles in the activation and maintenance of the checkpoint mechanisms that coordinate S phase and mitosis, and recent studies have unveiled its proto-oncogenic activity.
When does the synthesis of Cdc6 take place?
In any circumstance, when cells enter a new cell division cycle a new round of Cdc6 synthesis is required in order for pre-RCs assembly to occur. Our recent work suggests that Cdc6 also acts as a CDK1/Clb2 inhibitor at the end of mitosis, cooperating with Sic1 and APC/Hct1 in the inactivation of mitotic CDKs ( Fig. 12A) ( Calzada et al., 2001 ).
What is the function of cell division cycle 6?
Cell division cycle 6 (CDC6) is an essential regulator of DNA replication in eukaryotic cells. Its best-characterized function is the assembly of prereplicative complexes at origins of replication during the G 1 phase of the cell division cycle.