Which HLA typing gives high resolution matching?
In recent years, various HLA-typing methods using different NGS approaches have been performed. NGS-based HLA typing can provide high-resolution, unambiguous, phase-defined HLA alleles, avoiding several limitations compared to traditional sequence-based typing methods (Carapito et al., 2016).
What does HLA-DQB1 do?
The HLA-DQB1 gene is part of a family of genes called the human leukocyte antigen (HLA) complex. The HLA complex helps the immune system distinguish the body’s own proteins from proteins made by foreign invaders such as viruses and bacteria.
What is low resolution HLA typing?
Low resolution is a DNA-based typing result at the level of the first field (formerly 2-digit) in the DNA based nomenclature. If none of the above resolutions can be achieved, DNA-based low resolution typings are accepted.
What is DQB1 * 02?
HLA-DQ2 and HLA-DQB1*02 are almost synonymous in meaning. DQ2 β-chains combine with α-chains, encoded by genetically linked HLA-DQA1 alleles, to form the cis-haplotype isoforms. These isoforms, nicknamed DQ2. 2 and DQ2.
What does HLA-DQ2 positive mean?
If the test results are positive for HLA DQ2 or DQ8, your child is at risk of developing celiac disease. It doesn’t mean they will definitely have celiac disease and the chance of developing celiac disease is still low.
What does HLA-DQ8 positive mean?
Testing positive for either HLA-DQ2 or HLA-DQ8 does not mean you have to change your eating habits unless you have symptoms of the disease (including damage to the villi that line the small intestines). The test will simply tell you that you have a propensity for the disease, nothing more.
What is the most common HLA type?
Results. The most common alleles found in Caucasians were HLA-A*02, 24, 01; HLA-B*35, 44, 51; DRB1*11, 13, 07; for Afro-Brazilians they were HLA-A*02, 03, 30; HLA-B*35, 15, 44; DRB1*13, 11, 03; and for Asians they were: HLA-A*24, 02, 26; HLA-B*40, 51, 52; DRB1*04, 15, 09.
What does HLA-DQ2 5 positive mean?
In addition, there are at least three different versions of the HLA-DQ2 gene. One, known as HLA-DQ2. 5, confers the highest risk for celiac disease3; about 13% of Caucasian residents of the U.S. carry this specific gene. However, people with other versions of HLA-DQ2 also are at risk for celiac disease.
What kind of typing is used for HLA?
HLA class II (HLA-DRB1, DRB3, DRB4, DRB5, DQA1, DQB1, DPA1, and DPB1) intermediate resolution typing To determine HLA types of solid organ transplant recipient and donor candidates. To evaluate living and deceased donors for solid organ transplants.
What kind of sequencing is used for HLA?
Next-generation sequencing (NGS) is used to determine the whole HLA gene sequences including all exons and introns. Comprehensive NGS typing includes all eleven major histocompatibility HLA genes: HLA-A, B, C, DRB1, DRB3, DRB4, DRB5, DQA1, DQB1, DPA1, and DPB1.
How are HLA typing results reported in rsso?
Every sample is concurrently typed by rSSO for all 11 HLA loci to corroborate NGS HLA types and to confirm homozygosity. HLA typing results are reported up to three-field format (e.g., HLA-A*01:01:01). Comprehensive patient and donor typing report identifies the matched donor allele at each locus as well as total loci.
What are the 11 HLA genes in NGS?
Comprehensive NGS typing includes all eleven major histocompatibility HLA genes: HLA-A, B, C, DRB1, DRB3, DRB4, DRB5, DQA1, DQB1, DPA1, and DPB1. Every sample is concurrently typed by rSSO for all 11 HLA loci to corroborate NGS HLA types and to confirm homozygosity.