What is the molecular weight of beta-actin?
approximately 42 kDa
Applications. Beta actin is often used in Western blotting as a loading control, to normalize total protein amounts and check for eventual protein degradation in the samples. Its transcript is also commonly used as a housekeeping gene standard in qPCR. Its molecular weight is approximately 42 kDa.
What is the size of actin protein?
approximately 7 nm
The major cytoskeletal protein of most cells is actin, which polymerizes to form actin filaments—thin, flexible fibers approximately 7 nm in diameter and up to several micrometers in length (Figure 11.1).
What is beta-actin antibody?
β-Actin Antibody detects endogenous levels of β-actin. Due to the high sequence identity between the actin isoforms, β-Actin Antibody may cross-react with other actin isoforms.
What is the purpose of beta-actin?
Beta-actin, is usually used as a loading control for Western Blot to normalize the levels of protein detected by confirming that protein loading is the same across the gel.
What is the function of beta-actin?
Beta-Actin Protein They can be used to normalize the levels of protein detected by confirming that protein loading is the same across the gel. Beta-actin is an isform of actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity.
What does G actin stand for?
It exists in two forms: G-actin (monomeric globular actin) and F-actin (polymeric fibrous actin), the form involved in muscle contraction. When a signal for muscle contraction is sent along a nerve to a muscle cell, actin and myosin are activated.
Are actin filaments thick or thin?
Sarcomeres are highly stereotyped and are repeated throughout muscle cells, and the proteins within them can change in length, which causes the overall length of a muscle to change. An individual sarcomere contains many parallel actin (thin) and myosin (thick) filaments.
Where is G actin found?
The actin protein is found in both the cytoplasm and the cell nucleus. Its location is regulated by cell membrane signal transduction pathways that integrate the stimuli that a cell receives stimulating the restructuring of the actin networks in response.
What is the role of beta-actin?
Beta-actin, also known as a “housekeeping” protein, is usually used as a loading control , for among others, the integrity of cells, protein degradation, in PCR and Western blotting. They can be used to normalize the levels of protein detected by confirming that protein loading is the same across the gel.
What is the molecular weight of beta actin?
Though beta-actin has a molecular weight of 42 kDa, degradation will produce 35–38 kDa, ~ 30 kDa, 15 kDa, and other typical fragments on a Western blot, of which 35-38 kDa fragments are the most common.
Which is the best anti beta actin antibody?
All lanes : Anti-beta Actin antibody (ab8227) at 1/1000 dilution Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear lysate Lane 2 : HeLa whole cell lysate Lane 3 : A431 (human epidermoid carcinoma cell line) cell lysate Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Where are beta actin and gamma actin isoforms expressed?
Four of these isoforms are expressed primarily in striated and smooth muscle cells, whereas beta-actin and gamma-actin isoforms are ubiquitously expressed.
How is anti beta actin antibody loading control ( Abcam ) created?
The membrane was then blocked for an hour using 5% Bovine Serum Albumin (lanes 1-4) or 3% milk (lanes 5-8) before being incubated with ab8229 overnight at 4°C. Antibody binding was detected using an anti-Goat antibody conjugated to HRP, and visualised using ECL development solution.